induction of premature chromosome condensation (pcc) by calyculin a for biodosimetry

Authors

s. hosseini novin medical radiation institute, tehran, iran

h. mozdarani

abstract

background: premature chromosome condensation (pcc) is a method for interphase chromosome analysis in biodosimetry. this study was performed to verify the usefulness of pcc induced by calyculin a in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. materials and methods: peripheral blood was obtained from a healthy donor and exposed to various doses (0.25–4 gy) of γ -rays. the frequency of simple breaks and dicentrics were scored in g2/m chromosomes of giemsa stained cells. results: results show that the frequency of simple chromosome breaks appears to increase linearly with dose while the frequency of dicentrics apparently increases linear-quadratically with the dose. conclusion: induction of chromosome condensation by calyculin a is a powerful biodisimetric method, which provides a high number of spreads for analysis. with the use of this method, it is possible to overcome problems related to low mitotic index or cell-cycle alterations in routine metaphase analysis and low fusion rate in conventional pcc technique. iran . j. radiat. res., 2004 2 (1): 15-20

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry

Background: Premature chromosome condensation (PCC) is a method for interphase chromosome analysis in biodosimetry. This study was performed to verify the usefulness of PCC induced by calyculin A in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. Materials and Methods: Peripheral blood was obtained from a healthy donor and expo...

full text

Induction of Premature Chromosome Condensation (PCC) by Calyculin A for biodosimetry

B iological dosimetry of ionizing radiation is generally performed by scoring dicentrics and/or translocations in chromosome from peripheral blood lymphocytes (pbl) at the first mitosis following in vitro growth stimulation. It is well known that after irradiation, in this procedure mitotic index is low in some individuals, so the scored mitoses might not be representative of the exposed cell p...

full text

Biological Dose Assessment by Chemical Induction of Premature Chromosome Condensation

Dose assessment of radiation victims is a key element in medical management of radiation accidents. At high radiation doses (> 6 Gy), dicentric assay lose its efficiency and premature chromosome condensation (PCC) assay was proposed to overcome the restrictions of dicentric assay. It was shown that PCC ring is more suitable and simpler than dicentric for biodosimetry in high radiation doses. In...

full text

Construction of a dose – response curve by induction of premature chromosome condensation for biological dosimetry

Premature Chromosome Condensation (PCC) appears to have a possible utility for biological dosimetry purposes. The PCC technique may be adapted for cases of suspicion of overexposure where sampling is performed at least one day after an accident. For this purpose, human blood samples were exposed in vitro to 60Co up to 10 Gy and the PCC technique was performed immediately after irradiation. Anal...

full text

construction of a dose – response curve by induction of premature chromosome condensation for biological dosimetry

premature chromosome condensation (pcc) appears to have a possible utility for biological dosimetry purposes. the pcc technique may be adapted for cases of suspicion of overexposure where sampling is performed at least one day after an accident. for this purpose, human blood samples were exposed in vitro to 60co up to 10 gy and the pcc technique was performed immediately after irradiation. anal...

full text

Induction of premature chromosome condensation Premature chromosome condensation (PCC) was induced in HeLa cells using polyethylene glycol-mediated fusion of lectin-bound

The mammalian kinetochore is a specialized structure, found at the surface of the primary constriction of metaphase chromosomes, that plays a role in both the capture of spindle microtubules and movements associated with anaphase A (for a review, see Rattner, 1991). The kinetochore is first detected as an aggregate of fine fibrillar material associated with condensing chromatin in midto late-pr...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of radiation research

جلد ۲، شماره ۱، صفحات ۱۵-۲۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023